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A Macrophage‐Derived Factor That Inhibits the Production and Action of Interleukin 2
Author(s) -
Krakauer Teresa
Publication year - 1985
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.38.3.429
Subject(s) - biology , macrophage , action (physics) , production (economics) , interleukin , immunology , microbiology and biotechnology , cytokine , biochemistry , in vitro , physics , macroeconomics , quantum mechanics , economics
The human monocytic leukemia cell line THP‐1 produces an immunosuppressive factor that inhibits interleukin 1 (IL‐1)‐dependent proliferation of mouse thymocytes as well as the mitogenic effects of concanavalin A (Con A) and phytohemagglutinin (PHA) on human peripheral blood mononuclear cells. The mechanism of action of this factor includes interference with both the production of interleukin 2 (IL‐2) and its effects on target cells. Thus, the suppressor abrogates the proliferation of an IL‐2‐dependent cytotoxic T cell line (CTLL), but not of IL‐2 independent cells like the L929 fibroblasts or the EL4 T lymphoma and U937 histiocytic lymphoma lines. It also suppresses IL‐2 production by human peripheral blood enriched T cells and mouse splenocytes. The mediator has a molecular weight of 60,000–70,000 dalton, as determined by gel filtration chromatography, is heat labile, and is sensitive to trypsin, chymotrypsin, and protease.