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Effects of Recombinant Interferon‐γ on HLA‐DR Antigen Shedding by Human Peripheral Blood Adherent Mononuclear Cells
Author(s) -
Gershon Harriet E.,
Kuang YanDe,
Scala Giuseppe,
Oppenheim Joost J.
Publication year - 1985
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.38.2.279
Subject(s) - antigen , biology , peripheral blood mononuclear cell , microbiology and biotechnology , pan t antigens , antibody , immunology , hla dr , in vitro , monoclonal antibody , biochemistry
Two reproducible and sensitive assays have been developed for the detection of cell‐free HLA‐DR antigen, an antibody‐mediated complement‐dependent cytotoxicity inhibition assay (CIA) and a competition enzyme‐linked immunosorbent assay (CELISA). One unit of cell‐free HLA‐DR antigen has been quantitated to be the equivalent of 27.5 ng pure DR antigen/ml. Human peripheral blood adherent mononuclear cells (PBAMC), as well as DR + monocytes and B lymphoblastoid cell lines but not T lymphocytes, were observed to shed DR‐bearing vesicles in vitro. Human recombinant IFN‐γ induces the expression of Ia/DR antigen by PBAMC by increasing both the number of cells expressing DR antigen and the density per cell. After incubation with IFN‐γ, PBAMC also shed significantly more DR antigen. The degree of DR expression and shedding is dependent on the dose of IFN‐γ. The shedding of DR antigen occurred concomitantly with the inductive phase of cell membrane antigen expression, and very little DR antigen appeared in culture supernatants subsequent to the removal of IFN‐γ. The possible physiologic significance of shed DR antigen is discussed.