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Interferon Produced Endogenously in Response to CSF‐1 Augments the Functional Differentiation of Progeny Macrophages
Author(s) -
Moore Robert N.,
Pitruzzello Frank J.,
Robinson Richard M.,
Rouse Barry T.
Publication year - 1985
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.37.5.659
Subject(s) - interferon , biology , mononuclear phagocyte system , peripheral blood mononuclear cell , antiserum , colony stimulating factor , immunology , opsonin , phagocyte , macrophage , endogeny , phagocytosis , endocrinology , antigen , in vitro , microbiology and biotechnology , haematopoiesis , biochemistry , stem cell
The role of endogenously produced interferon α/ β in the functional maturation of newly derived mononuclear phagocytes was investigated. Addition of highly specific anti‐interferon α+ βantiserum to murine marrow cultures stimulated with colony‐stimulating factor‐1 (macrophage growth factor) markedly suppressed the capacity of resulting progeny mononuclear phagocytes to ingest opsonized sheep erythrocytes (EA lgG ). This impairment was corrected either by direct addition of interferon α+ βat a concentration in excess of that neutralized by the antiserum or by the addition of lesser amounts of interferon (33 U/ml) following removal of the anti‐interferon from the cultures. Conditioned media from control colony‐stimulating factor‐stimulated cultures similarly reversed the impairment of maturation resulting from 5 days of growth in the presence of anti‐interferon. This enhancement of EA |gG ingestion reflected upon the interferon activity in the conditioned media and was neutralized by anti‐interferon. Lastly, the endogenous interferon was found to enhance EA lgG ingestion by a majority of the mononuclear phagocyte progeny and not by a limited subpopulation.