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Cultured Human Alveolar Macrophages From Smokers With Lung Cancer: Resolution of Factors That Stimulate Fibroblast Proliferation, Production of Collagenase, or Prostaglandin E 2
Author(s) -
Dayer J.M.,
Sundstrm L.,
Polla B.S.,
Junod A.F.
Publication year - 1985
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.37.5.641
Subject(s) - collagenase , fibroblast , prostaglandin e2 , biology , bronchoalveolar lavage , macrophage , connective tissue , lung , monocyte , immunology , alveolar macrophage , pulmonary alveolus , pathology , microbiology and biotechnology , in vitro , endocrinology , medicine , biochemistry , genetics , enzyme
Marked connective tissue remodelling involves both destruction and repair in inflammatory lung diseases. Throughout the remodelling event, it was reasoned that alveolar macrophages may release substances similar to those produced by blood monocyte‐macrophages that affect fibroblast functions, ie, the interleukin 1 family of monokines (or cytokines). We have examined human alveolar macrophage cultures obtained after bronchoalveolar lavage of freshly excised lungs from heavy smokers with bronchial carcinoma. Crude culture media contained fibroblast proliferative activity and collagenase‐ and PGE 2 ‐ production‐stimulating activity. The main peak of these biological activities was located around ‐ I8 kilodaltons (kD) on gel filtration chromatography. Resolution of this peak by high performance liquid chromatography showed the presence of three distinct peaks, with quantitative and qualitative differences in biological activities. This suggests the presence of heterogeneous factors.