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Interferon Production in Murine Macrophage‐Like Cell Lines
Author(s) -
Salo Richard J.,
Bleam Diana K.,
Greer Veronica L.,
Ortega Andrew P.
Publication year - 1985
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.37.4.395
Subject(s) - biology , macrophage , cell culture , interferon , microbiology and biotechnology , interferon gamma , immunology , virology , cytokine , genetics , in vitro
Five murine macrophage (MΦ)‐like cell lines were examined to determine their suitability for the characterization of MΦ interferons (IFNs). The J774A.1, RAW 309 Cr.1, and RAW 264.7 cell lines produced 30–800 international units (IU)/ 10 6 cells when treated with 5–200 μg of bacterial lipopolysaccharide (LPS). No IFN was detected in LPS‐treated P388D 1 or PU5‐1.8 cell cultures. All cell lines produced IFN when inoculated with Newcastle disease virus (NDV); however, only 15 IU/10 6 cells of acid stable IFN were produced in PU5‐1.8 cell cultures in comparison to 4.2 × 10 3 ‐1.7 × 10 4 IU/10 6 cells in the other cell lines. Most of the IFN was produced within 4 h in LPS‐treated cell cultures and within 12 h in NDV‐infected cell cultures. All IFNs were stable at pH 2.0 and were neutralized with antiserum against mouse L cell IFN. These cell lines appear competent for use in studying the synthesis, molecular weights, and regulatory functions of MΦ IFNs.