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Enhancement of Hamster Alveolar Macrophage Phagocytic Activity by Lymphokines
Author(s) -
Widen Raymond H.,
Paradise Lois J.
Publication year - 1985
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.37.3.349
Subject(s) - lymphokine , concanavalin a , splenocyte , pulmonary alveolus , hamster , biology , phagocytosis , macrophage , macrophage activating factor , incubation , immunology , alveolar macrophage , microbiology and biotechnology , immune system , in vitro , biochemistry
Modulation of phagocytic activity of resident hamster pulmonary alveolar macrophages was accomplished by incubation of the cells in lymphokines prepared by stimulation of hamster splenocytes with concanavalin A or alloantigens in mixed lymphocyte cultures. Alveolar macrophages preincubated in either of these lymphokine preparations possessed significantly greater ability to ingest IgG or IgM plus complement‐coated sheep erythrocytes, via their Fc or complement receptors, respectively, than macrophages exposed to control preparations. Ingestion of yeast particles also was enhanced with macrophages incubated in supernatants from cultures of stimulated splenocytes. Supernatant fluids from either mitogen‐ or alloantigen‐stimulated splenocytes possessed migration inhibitory activity with characteristics similar to MIF from other animals; the phagocytosis‐enhancing activity shared some of these characteristics.