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Induction of Neovascularization and Nonlymphoid Mesenchymal Cell Proliferation by Macrophage Cell Lines
Author(s) -
Polverini Peter J.,
Leibovich S. Joseph
Publication year - 1985
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.37.3.279
Subject(s) - biology , mesenchymal stem cell , macrophage , neovascularization , microbiology and biotechnology , cell culture , cell growth , cell , cancer research , immunology , angiogenesis , genetics , in vitro
The mature murine macrophage‐like cells NCTC‐3749 and J‐774, the Immature human macrophage‐like cells U‐937‐1, and their conditioned media exhibited potent angiogenic activity in rat corneas and stimulated proliferation of bovine aortic endothelial cells (BAEC) and DNA synthesis in BALB/C‐3T3 cells in culture. In contrast, the immature human macrophage‐like cells HL‐60 and their conditioned media either failed to produce or release detectable quantities of these activities. Exposure of HL‐60 cells to phorbol‐myristate‐acetate (PMA) did not enhance expression of angiogenic and growth stimulating activities by these cells. Both the angiogenic and growth stimulating activities appear to be mediated by a factor(s) that has biochemical properties in common with macrophage‐derived growth factor (MDGF) produced by normal rat peritoneal macrophages. These results suggest that NCTC‐3749, J‐774, and U‐937‐1 macrophage‐like cell lines may be a useful source for the large scale production and characterization of MDGF and macrophage‐derived angiogenic activity.