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Influence of Prostaglandin I 2 on Fibronectin‐Mediated Phagocytosis In Vivo and In Vitro
Author(s) -
Weinberg David A.,
Weston Lisa K.,
Kaplan John E.
Publication year - 1985
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.37.2.151
Subject(s) - phagocytosis , biology , in vitro , in vivo , fibronectin , prostaglandin , prostaglandin e2 , microbiology and biotechnology , immunology , biochemistry , endocrinology , extracellular matrix
This study evaluated the effect of prostaglandin I 2 (PGI 2 )on fibronectin‐me‐ diated macrophage phagocytosis in vivo and in vitro. Phagocytosis measured in vivo in rats by the vascular clearance rate and hepatic localization of gelatinized sheep erythrocytes was inhibited in a dose‐dependent manner after intravenous administration of PGI 2 . Phagocytosis was assessed in vitro in terms of uptake of fibronectin‐dependent gelatinized sheep erythrocytes by monolayers of casein‐elicited rat peritoneal macrophages. Concentrations of 1 ng/ml PGI 2 or greater resulted in inhibition of particle internalization but not attachment to macrophages. This inhibitory effect was enhanced by amino‐ phylline, a phosphodiesterase inhibitor. PGI 2 increased cAMP levels and these were further increased in the presence of aminophylline. These data indicate that PGI 2 inhibits macrophage uptake of gelatinized particles and support the idea that this is mediated by increased intracellular levels of cyclic AMP. PGI 2 should thus be considered a potential etiologic factor in the phagocytic depression observed in association with thrombosis.