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Macrophage‐Mediated Tumor Lysis Induced by Loach Egg Lectin
Author(s) -
Yamazaki Masatoshi,
Okutomi Takafumi,
Sakakibara Fusao,
Kawauchi Hiroaki,
Mizuno Den'ichi
Publication year - 1984
Publication title -
journal of leukocyte biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.819
H-Index - 191
eISSN - 1938-3673
pISSN - 0741-5400
DOI - 10.1002/jlb.35.3.241
Subject(s) - biology , cytolysis , lectin , macrophage , lysis , microbiology and biotechnology , spleen , lipopolysaccharide , immunology , biochemistry , cytotoxicity , in vitro
Vertebrate lectin purified from loach egg was tested for induction of tumor lysis mediated by macrophages. Loach egg lectin lysed tumor cells but not normal spleen cells in cooperation with BCG‐ or glucan (TAK)‐elicited peritoneal macrophages of mice. Corynebacterium parvum‐, OK432‐, glycogen‐, lipopolysaccharide‐elicited or resident macrophages were not effective. Neither loach egg lectin nor BCG nor glucan macrophages alone had a cytolytic action on tumor cells. Thus, the vertebrate lectin from loach egg is a mediator in macrophage‐mediated tumor lysis, inducing binding of macrophages to target cells. This lectin‐dependent macrophage‐mediated cytolysis (LDMC) was inhibited by galactose, N‐acetylgalactosamine, fucose, or rhamnose. These results suggest that tumor cells can be recognized via glycoconjugates on cell membrane in addition to tumor‐associated antigen and that some animal lectins participate in macrophage‐mediated tumor lysis.