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Targeting RNA with Conformationally Restricted Oligonucleotides
Author(s) -
Herdewijn Piet
Publication year - 1996
Publication title -
liebigs annalen
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 0947-3440
DOI - 10.1002/jlac.199619960902
Subject(s) - phosphoramidate , oligonucleotide , chemistry , nucleic acid , rna , complementarity (molecular biology) , duplex (building) , nucleic acid structure , computational biology , stereochemistry , biochemistry , dna , genetics , gene , biology
A configurational and conformational complementarity between targeted ssRNA and the antisense oligonucleotide is a prerequisite for efficient hybridization. In the design of oligonucleotides that may hybridize strongly with natural RNA, one may select molecules with a preorganized structure. The hybridization process should then benefit from less negative entropy changes during duplex formation. Although, it is by now not completely clear which conformational restricted motif is best suited for this purpose, the N3′–P5′ phosphoramidate linkage and the hexitol nucleic acids seems to gain the objective.