Fluorogenic Substrates Containing 7‐Amino‐4‐methyl‐2‐quinolinone for Aminopeptidase M, Chymotrypsin, Elastase and Trypsin, Determination of Enzyme Activity

Several new fluorogenic substrates for four proteases were synthesized by coupling the highly fluorescent 7 ‐amino‐4‐methyl‐2‐quinolinone (AMeq) with appropriate amino acids and peptides. Compounds HCl · H‐Ala‐NH‐Meq ( 1 ) and HCl · H‐Leu‐NH‐Meq ( 2 ) are substrates for aminopeptidase M, Glt‐Tyr‐NH‐Meq ( 3 ) and Glt‐Leu‐Tyr‐NH‐Meq ( 4 ) for chymotrypsin, Boc‐(Ala) 4 ‐NH‐Meq ( 5 ) for elastase and Z‐Arg‐NH‐Meq ( 6 ) for trypsin. The kinetic parameters for the hydrolysis of the new substrates were determined and showed that each new substrate is suitable for a convenient fluorometric assay of the corresponding enzyme.