Premium
Multidimensional NMR Techniques for the Elucidation of Oligosaccharide Structures: Verbascosaponin and a Further Saponin from Wool Flower
Author(s) -
Schröder Harald,
Haslinger Ernst
Publication year - 1993
Publication title -
liebigs annalen der chemie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 0170-2041
DOI - 10.1002/jlac.1993199301153
Subject(s) - chemistry , saponin , oligosaccharide , wool , stereochemistry , biochemistry , medicine , alternative medicine , pathology , archaeology , history
The application of 1D selective, 2D and 3D NMR techniques to the structure elucidation of verbascosaponin ( 1 ) is demonstrated. Combinations of ROESY and TOCSY are powerful tools. Each of these multidimensional ROESY‐TOCSY and TOCSY‐ROESY experiments provides detailed information about the sequence of oligosaccharide structures and the positions of glycosidation. The structure of a further saponin 2 from the officinal drug Flores Verbasci is determined as 3β‐ O ‐{α‐ L ‐rhamnopyranosyl‐(1→4)‐β‐ D ‐glucopyranosyl‐(1→3)‐[β‐ D ‐glucopyranosyl‐(1→2)‐]‐β‐ D ‐fucopyranosyl}‐13β,28‐epoxyolea‐11‐ene‐16α,23α‐diol.