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Preparative‐enzymatic formation of cytidine 5′‐monophosphosialate by integrated cytidine 5′‐triphosphate regeneration
Author(s) -
Thiem Joachim,
Stangier Peter
Publication year - 1990
Publication title -
liebigs annalen der chemie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 0170-2041
DOI - 10.1002/jlac.1990199001199
Subject(s) - cytidine , chemistry , nucleotide salvage , neuraminic acid , enzyme , uridine , biochemistry , adenylate kinase , nucleotide , rna , glycoprotein , gene
The preparative formation of cytidine 5′‐triphosphate by an enzymatic process with adenylate kinase and pyruvate kinase is described. The enzymes may either be used free, in the “MEEC” technique, or immobilized on a synthetic copolymer of vinyl acetate and N , N ′‐divinylethyleneurea (“VA‐Epoxy”). Cytidine 5′‐monophosphosialate synthase (preferably isolated from calf brain) and inorganic pyrophosphatase were used to prepare the activated neuraminic acid cytidine 5 ′‐monophosphosialate (CMP‐Neu5Ac) from CTP and neuraminic acid in good yield. Finally, both processes could be integrated and led to an in situ approach which, owing to moderate yields, requires further development.