Premium
Eine neue Synthese von Thymosin α 1
Author(s) -
Echner Hartmut,
Voelter Wolfgang
Publication year - 1988
Publication title -
liebigs annalen der chemie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 0170-2041
DOI - 10.1002/jlac.198819881115
Subject(s) - chemistry , thymosin , peptide , chromatography , reagent , acetylation , peptide synthesis , high performance liquid chromatography , amino acid , solid phase synthesis , biochemistry , organic chemistry , gene
A New Synthesis of Thymosin α 1 Thymosin α 1 , an N ‐acetylated octacosa thymus peptide, isolated first from thymosin fraction 5, is synthesized by the solid phase method using the p ‐benzyloxybenzyl alcohol/polystyrene/divinylbenzene resin, N α ‐Fmoc‐amino acids and those with tert ‐butyl or Boc side chain protection. All couplings are performed with the Bop reagent. The peptide is purified by a combination of gel chromatography and preparative HPLC, its purity checked by amino acid analysis and analytical HPLC, and the biological activity tested by the E‐rosette assay.