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Synthesis of Deoxyoligonucleotide Linker Fragments for Genetic Engineering Using Improved Preparative and Analytical Techniques
Author(s) -
Seliger Hartmut,
Bach TrungChinh,
Siewert Gerhard,
Boidol Werner,
Töpert Michael,
Schulten HansRolf,
Schiebel Hans Martin
Publication year - 1984
Publication title -
liebigs annalen der chemie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 0170-2041
DOI - 10.1002/jlac.198419840502
Subject(s) - cyanogen bromide , linker , chemistry , cleavage (geology) , combinatorial chemistry , dna , mass spectrometry , chromatography , biochemistry , peptide sequence , computer science , geotechnical engineering , fracture (geology) , engineering , gene , operating system
For studies on the expression of recombinant DNA, linker fragments have been prepared which serve to introduce specific points of cleavage on the DNA as well as the protein level. Such linkers were designed for cyanogen bromide as well as for collagenase cleavage of fused proteins. The preparation was done according to the triester synthesis scheme using improved and simpler routes to functionalized dinucleotide building blocks. Field desorption mass spectrometry was used as a routine tool for the identification and control of the purity of these units.