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Chromatographic Separation and Spectroscopic Characterization of the Bilirubin Isomers IIIα, IXα, XIIIα and Their Dimethyl Esters
Author(s) -
DefoinStraatmann Rosalie,
Defoin Albert,
Kuhn Hans Joachim,
Schaffner Kurt
Publication year - 1982
Publication title -
liebigs annalen der chemie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.825
H-Index - 155
eISSN - 1099-0690
pISSN - 0170-2041
DOI - 10.1002/jlac.198219821002
Subject(s) - chemistry , chromatographic separation , chromatography , characterization (materials science) , high performance liquid chromatography , organic chemistry , nanotechnology , materials science
The three structural isomers bilirubin IXα ( 1a ), IIIα ( 2a ), and XIIIα ( 3a ) have been separated on a preparative scale by high‐performance low‐pressure liquid chromatography. They were converted into their dimethyl esters 1b, 2b , and 3b and characterized by UV, IR, MS, and fully assigned 1 H NMR data. NMR is the only spectroscopic method which makes it possible to analyze mixtures of the three isomeric dimethyl esters quantitatively.