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Application of response surface design to optimise the chromatographic analysis of volatile compounds in beer
Author(s) -
Nešpor Jakub,
Karabín Marcel,
Hanko Vojtěch,
Dostálek Pavel
Publication year - 2018
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/jib.493
Subject(s) - chromatography , central composite design , chemistry , solid phase microextraction , extraction (chemistry) , gas chromatography , mass spectrometry , response surface methodology , gas chromatography–mass spectrometry , brewing , analyte , calibration curve , detection limit , organic chemistry , fermentation
Solid‐phase microextraction (SPME) combined with gas chromatography and mass spectrometry (GC–MS) is a rapid method for the analysis of different aromatic compounds in beer. However, chromatographic systems are affected by different parameters and optimization is time‐consuming process, but essential for establishing optimal conditions for the quantification of analytes. An automated system consisting of headspace (HS)–SPME extraction combined with GC–MS was optimized for the determination of 19 volatile compounds responsible for important flavours and off‐flavours of beer. The optimisation process consisted of two steps: the SPME fibre type was chosen, and subsequently four extraction parameters (temperature, time, sodium chloride concentration and pH) were optimised by a central composite design model. After optimisation, standard compounds were validated with relative standard deviations not exceeding 15% rel . The square of correlation coefficient for the calibration curves was ≥0.9559, indicating a linear response and the suitability of these HS‐SPME conditions. Copyright © 2018 The Institute of Brewing & Distilling