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Antiproliferative effects of prenylflavonoids from hops on human colon cancer cell lines
Author(s) -
Hudcová Tereza,
Bryndová Jana,
Fialová Kristýna,
Fiala Jaromír,
Karabín Marcel,
Jelínek Lukáš,
Dostálek Pavel
Publication year - 2014
Publication title -
journal of the institute of brewing
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.523
H-Index - 51
eISSN - 2050-0416
pISSN - 0046-9750
DOI - 10.1002/jib.139
Subject(s) - xanthohumol , chemistry , ic50 , brewing , cell culture , biochemistry , hop (telecommunications) , antioxidant , food science , in vitro , biology , fermentation , genetics , computer network , computer science
In recent years, interest in hop‐derived constituents, especially for prenylflavonoids has grown, as they have a wide range of biological properties including antioxidant, anticarcinogenic and antimicrobial activities. Two main hop prenylflavonoids, xanthohumol and isoxanthohumol, and hop extract enriched in prenylflavonoids, were tested for their antiproliferative activities on colon cancer cell lines, HT‐29 and SW620, and a noncancerous cell line, IEC‐6. It was confirmed that both xanthohumol and isoxanthohumol inhibited cell proliferation, even at micromolar concentrations. For cell line HT‐29, the IC 50 was 1.2 ± 0.9 and 16.9 ± 0.9 µmol dm −3 for xanthohumol and isoxanthohumol, respectively. Similar values were obtained for SW620 cells (2.5 ± 0.2 and 37.3 ± 3.2 µmol dm –3 ). None of the pure prenylflavonoids that were tested affected the proliferation of the noncancerous cell line, IEC‐6. The effect of the hop extract containing xanthohumol was also tested for antiproliferative activities on the cancer cell lines, HT‐29 (IC 50 = 3.1 ± 0.2 µmol dm –3 ) and SW620 (IC 50 = 1 ± 0.2 µmol dm −3 ), and on the cell line, IEC‐6 (IC 50 = 65.5 ± 11.3 µmol dm −3 ). The results showed a similar trend to that for pure compounds, suggesting a possible future application of hop extracts in the pharmaceutical industry. Copyright © 2014 The Institute of Brewing & Distilling