Synthesis and Biological Activity of Some New Pyrazole Copper(II) Complexes

As a part of systematic investigation of synthesis and biologically active compounds of pyrazole derivatives containing transition metal, several new pyrazole copper(II) complexes 3a−f were synthesized from pyrazole sodium salts 2a−f , which were produced from spiro‐pyrazoles 1a−f and sodium hydride by a ring‐opening reaction. All the synthesized compounds were characterized by spectroscopic analysis. Pyrazole copper(II) complexes 3a−d and 3f exhibited high DNA cleavage activity in vitro . Furthermore, compounds 3a−f were tested for their growth inhibitory activity in A549 lung cancer, B16F10 murine melanoma, and HeLa human uterine carcinoma cells. Compounds 3c,d displayed moderate B16F10 and HeLa inhibitory activity levels ( 3c : IC 50 = 45 μ M in B16F10 cells and 34 μ M in HeLa cells, 3d : IC 50 = 50 μ M in B16F10 cells and 32 μ M in HeLa cells).