Nonclassical 5‐substituted tetrahydroquinazolines as potential inhibitors of thymidylate synthase

Classical inhibitors of thymidylate synthase such as N l0 ‐propargyl‐5,8‐dideazafolic acid (1), N ‐(5‐[N‐(3,4‐dihydro‐2‐methyl‐4‐oxoquinazolin‐6‐ylmethyl)‐ N ‐methylamino]‐2‐thenoyl)‐L‐glutamic acid (ZD1694, 2) and N ‐[2‐amino‐4‐oxo‐3,4‐dihydro(pyrrolo[2,3‐ d ]pyrintidin‐5‐yl)ethylbenzoyl]‐L‐glutamic acid (LY231514, 3) while potent, suffer from a number of potential disadvantages, such as impaired uptake due to an alteration of the active transport system required for their cellular uptake, as well as formation of long acting, non‐effluxing polyglutamates via the action of folylpolyglutamate synthetase, which are responsible for toxicity. To overcome some of the disadvantages of classical inhibitors, there has been considerable interest in the synthesis and evaluation of nonclassical thymidylate synthase inhibitors, which could enter cells via passive diffusion. In an attempt to elucidate the role of saturation of the B‐ring of non‐classical, quinazoline antifolate inhibitors of thymidylate synthase, analogues 7‐17 were designed. Analogues 13‐17 which contain a methyl group at the 7‐position, were synthesized in an attempt to align the methyl group in an orientation which allows interaction with tryptophan‐80 in the active site of thymidylate synthase. The synthesis of these analogues was achieved via the reaction of guanidine with the appropriately substituted cyclohexanone‐ketoester. These ketoesters were in turn synthesized via a Michael addition of the appropriate thiophenol with 2‐carbethoxycyclohexen‐1‐one or 5‐methyl‐2‐carbethoxycyclo‐hexen‐1‐one to afford a mixture of diastereomers. The most inhibitory compound was the 3,4‐dichloro, 7‐methyl derivative 17 which inhibited the Escherichia coli and Pneumocystis carinii thymidylate syntheses 50% at 5 × 10 5 M. Our results confirm the importance of the 7‐CH 3 group and electron withdrawing groups on the aromatic side chain for thymidylate synthase inhibition.