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Decrease in major secondary bile acid, hyodeoxycholic acid, was the main alteration in hepatic bile acid compositions in a hypertensive nonalcoholic fatty liver disease model
Author(s) -
Kodama Masanobu,
Kanno Keishi,
Kishikawa Nobusuke,
Takei Hajime,
Nittono Hiroshi,
Tazuma Susumu
Publication year - 2019
Publication title -
journal of hepato‐biliary‐pancreatic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.63
H-Index - 60
eISSN - 1868-6982
pISSN - 1868-6974
DOI - 10.1002/jhbp.678
Subject(s) - medicine , bile acid , steatosis , nonalcoholic fatty liver disease , endocrinology , ursodeoxycholic acid , chemistry , fatty liver , disease
Background Previous findings on hepatic bile acid compositions in nonalcoholic fatty liver disease (NAFLD) have been inconsistent and complicated. The aim of this study was to investigate the effects of steatosis on hepatic bile acid composition in a hypertensive NAFLD model without obesity and diabetes mellitus and compare hepatic bile acid composition between hypertensive rats with and without steatosis. Methods Two groups of hypertensive rats were studied: spontaneously hypertensive rats (SHR) fed with a normal diet (SHR‐N) or a choline‐deficient diet (SHR‐CD). Two groups of normotensive rats were studied: Wistar Kyoto rats (WKY) fed a normal diet (WKY‐N) or a choline‐deficient diet (WKY‐CD). Hepatic bile acid analysis was performed using liquid chromatography–electrospray ionization–tandem mass spectrometry. Results Regarding bile acid composition, the hyodeoxycholic acid (HDCA) species in the SHR‐CD group showed the largest change in bile acid composition, significantly decreasing to 21.9% of that found in the SHR‐N group. In the WKY‐CD group, no reduction of HDCA species was observed. Conclusions We demonstrated that the decrease in HDCA species was the main alteration in a hypertensive NAFLD model. It was suggested that the decrease in HDCA species in the SHR‐CD group was caused by dysbiosis.