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Homing effect of adipose‐derived stem cells to the injured liver: the shift of stromal cell‐derived factor 1 expressions
Author(s) -
Saito Yu,
Shimada Mitsuo,
Utsunomiya Tohru,
Ikemoto Tetsuya,
Yamada Shinichiro,
Morine Yuji,
Imura Satoru,
Mori Hiroki,
Arakawa Yusuke,
Kanamoto Mami,
Iwahashi Shuichi,
Takasu Chie
Publication year - 2014
Publication title -
journal of hepato‐biliary‐pancreatic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.63
H-Index - 60
eISSN - 1868-6982
pISSN - 1868-6974
DOI - 10.1002/jhbp.147
Subject(s) - adipose tissue , transplantation , stem cell , homing (biology) , stromal cell , pathology , medicine , stromal cell derived factor 1 , liver transplantation , parenchyma , biology , immunology , chemokine , inflammation , cxcr4 , microbiology and biotechnology , ecology
Background Whether systemically transplanted human adipose‐derived stem cells ( ADSC s) homed to the injured liver in nude mice under stress with subsequent hepatectomy ( H x) and ischemia‐reperfusion ( I / R ) was investigated in the present study. The types of cells in the liver that were involved in the homing of ADSC s were clarified, with focus on the stromal‐derived factor‐1 ( SDF ‐1)/ C ‐ X ‐ C chemokine receptor type 4 ( CXCR ‐4) axis. Methods Adipose‐derived stem cells were transplanted intravenously immediately after 70% H x and I / R . ADSC s were traced by in vivo imaging for 24 h after transplantation and ADSC s were histologically detected in the liver. SDF ‐1 and CXCR ‐4 expressions in the liver were evaluated by real time RT‐PCR . The immunohistochemical analysis of SDF ‐1 was also performed to identify SDF ‐1 expressing cells in the liver. Results Adipose‐derived stem cells were found in various organs immediately following transplantation and almost accumulated in remnant liver or spleen at 6 h after transplantation. ADSCs were also histologically revealed in the harvested liver. H x and I / R injury significantly enhanced SDF ‐1 expressions regardless of ADSC s transplantation, and only ADSC transplantation increased CXCR ‐4 expressions. The predominant SDF ‐1 positive cells in the liver were equally identified in parenchymal and non‐parenchymal cells at 6 h, but shifted to non‐parenchymal cells at 24 h after transplantation. Conclusions Systemically transplanted ADSC s homed to the injured liver after transplantation, possibly based on the mechanisms of SDF ‐1/ CXCR ‐4 axis. Therefore, systemic transplantation might be an effective and practical route for the transplantation of ADSC s.