Open AccessImmune reconstitution following umbilical cord blood transplantation: IRES, a study of UK paediatric patients
Author(s) -
Girdlestone John,
Raymond Meera,
Shaw Bronwen,
Tulpule Sameer,
Devlia Vikesh R.,
Danby Robert,
Ahyee Trudy,
Saudemont Aurore,
Hough Rachael,
Veys Paul,
Ruggeri Annalisa,
Vora Ajay,
Marks David I.,
Gibson Brenda,
Wynn Robert,
Madrigal Alejandro,
Navarrete Cristina V.
Publication year - 2020
Publication title -
ejhaem
Language(s) - English
Resource type - Journals
ISSN - 2688-6146
DOI - 10.1002/jha2.12
Subject(s) - umbilical cord , umbilical cord blood transplantation , medicine , transplantation , immune system , immunology , surgery , hematopoietic stem cell transplantation
Abstract To obtain a qualitative as well as quantitative view immune reconstitution following umbilical cord blood (UCB) transplantation of paediatric patients, we utilised a broad panel of flow cytometry markers to monitor the phenotypes of lymphoid and myeloid cells at 1‐12 months post‐transplant. Samples were received from 46 patients with a median age of 3.3 years and survival was 76% at 1 year. Monocytes were at similar or higher median levels than in adult controls at all times tested, with a high CD16+ proportion in the first 3 months. NK cells were also within adult ranges, with a CD56++ high proportion in the first 6 months. B cell recovery was seen from 2 months in most patients and T cells from 3 months, both were delayed with anti‐thymocyte globulin (ATG) treatment. CD4:CD8 ratios were high in the first 6 months, and the proportion of T cells with recent thymic emigrant and naïve phenotypes rose from 3 months. NK and plasmacytoid dendritic cell numbers remained at reduced levels in patients not surviving to 1 year. Our results can serve as a useful reference for detailed monitoring of immune reconstitution in paediatric recipients of UCB.