Open AccessFidelity of peripheral blood for monitoring genomics and tumor immune‐microenvironment in myelodysplastic syndromes
Author(s) -
Lee SungEun,
Wang Feng,
TrujilloOcampo Abel,
RuizVasquez Wilfredo,
Cho HyunWoo,
Takahashi Koichi,
Molldrem Jeffrey J.,
Futreal Andrew,
GarciaManero Guillermo,
Im Jin S.
Publication year - 2020
Publication title -
ejhaem
Language(s) - English
Resource type - Journals
ISSN - 2688-6146
DOI - 10.1002/jha2.112
Subject(s) - immune system , bone marrow , peripheral blood , myelodysplastic syndromes , tumor microenvironment , concordance , biology , immunology , medicine , cancer research , bioinformatics
The aim of this study is to investigate whether the peripheral blood (PB) can serve as a surrogate immune‐microenvironment to bone marrow for genetic and immune monitoring in myelodysplastic syndrome (MDS). We compared the composition of T cell subsets and somatic mutation burden in 36 pairs of PB and matching bone marrow aspirate (BMA) using multi‐parameter flow cytometry and NGS‐based targeted sequencing analysis, respectively. Our immune‐subset and NGS‐based mutation analysis of BMA showed significant concordance with those of PB in MDS. Therefore, PB can provide easily accessible tumor immune‐microenvironment for monitoring in the immune and genetic landscapes for MDS patients.