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In vivo DNA gene electro‐transfer: a systematic analysis of different electrical parameters
Author(s) -
Zampaglione Immacolata,
Arcuri Mirko,
Cappelletti Manuela,
Ciliberto Gennaro,
Perretta Gemma,
Nicosia Alfredo,
La Monica Nicola,
Fattori Elena
Publication year - 2005
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.774
Subject(s) - electroporation , transduction (biophysics) , plasmid , gene , in vivo , gene delivery , genetic enhancement , reporter gene , dna vaccination , gene transfer , biology , dna , computational biology , microbiology and biotechnology , genetics , gene expression , biophysics
Background Intramuscular plasmid injection followed by electroporation is an efficient method for gene therapy or vaccination. Several protocols have been described that give good transduction levels with several reporter genes. Methods In this work we have explored the efficiency of gene delivery upon variation of the different electrical parameters such as pulse length frequency and voltage monitoring both on short‐ and long‐term protein production. Results Having defined the best performing parameters, we have designed a short electric treatment that gives good levels of plasmid‐encoded protein in different species such as mice, rabbits and monkeys. Copyright © 2005 John Wiley & Sons, Ltd.