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Sendai virus vectors as an emerging negative‐strand RNA viral vector system
Author(s) -
Bitzer Michael,
Armeanu Sorin,
Lauer Ulrich M.,
Neubert Wolfgang J.
Publication year - 2003
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.426
Subject(s) - sendai virus , biology , transduction (biophysics) , virology , vector (molecular biology) , virus , viral vector , viral replication , gene , rna virus , rna , genetics , biochemistry , recombinant dna
The power to manipulate the genome of negative‐strand RNA viruses, including the insertion of additional non‐viral genes, has led to the development of a new class of viral vectors for gene transfer approaches. The murine parainfluenza virus type I, or Sendai virus (SeV), has emerged as a prototype virus of this vector group, being employed in numerous in vitro as well as animal studies over the last few years. Extraordinary features of SeV are the remarkably brief contact time that is necessary for cellular uptake, a strong but adjustable expression of foreign genes, efficient infection in the respiratory tract despite a mucus layer, transduction of target cells being independent of the cell cycle, and an exclusively cytoplasmic replication cycle without any risk of chromosomal integration. In this review we describe the current knowledge of Sendai virus vector (SeVV) development as well as the results of first‐generation vector applications under both in vitro and in vivo conditions. So far, Sendai virus vectors have been identified to be a highly efficient transduction tool for a broad range of different tissues and applications. Future directions in vector design and development are discussed. Copyright © 2003 John Wiley & Sons, Ltd.