Premium
PP7080 expedites the proliferation and migration of lung adenocarcinoma cells via sponging miR‐670‐3p and regulating UHRF1BP1
Author(s) -
Dan Weibin,
Shi Lei,
Wang Li,
Wu Dahe,
Huang Xiaofang,
Zhong Yong
Publication year - 2021
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.3341
Subject(s) - cancer research , adenocarcinoma , chemistry , microbiology and biotechnology , medicine , biology , cancer
Background An increasing body of evidence has revealed that long non‐coding RNAs play a significant part in a variety of human cancers, including lung adenocarcinoma (LUAD). Methods The expression of PP7080, miR‐670‐3p and UHRF1BP1 in LUAD cells and tissues was detected using a quantitative real‐time polymerase chain reaction. The role of PP7080 in LUAD cells was validated by CCK‐8, flow cytometry, colony formation, transwell and wound healing assays. The binding capacity between PP7080/UHRF1BP1 and miR‐670‐3p was confirmed by luciferase reporter assays. Moreover, the interactional mechanism among PP7080, miR‐670‐3p and UHRF1BP1 was determined by means of RNA immunoprecipitation and western blot assays. Results The expression level of PP7080 is up‐regulated in LUAD cells and tissues compared to their matched controls. Down‐regulation of PP7080 restrained the proliferative and migratory abilities of LUAD cells, but induced cell apoptosis. PP7080 up‐regulation led to the opposite results. Moreover, the binding ability between miR‐670‐3p and PP7080/UHRF1BP1 in LUAD cells was confirmed. A rescue assay revealed that PP7080 contributes to LUAD development by modulating the miR‐670‐3p/UHRF1BP1 signaling pathway. Conclusions PP7080 expedites the proliferation and migration of LUAD cell via sponging miR‐670‐3p and modulating UHRF1BP1.