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Variants in TNF and NOS3 ( eNOS ) genes associated with sepsis in adult patients
Author(s) -
Özkan Mustafa,
Günay Nurullah,
Sener Elif Funda,
Karcıoglu Özgür,
Tahtasakal Reyhan,
Dal Fatma,
Günay Nahide Ekici,
Demiryürek Abdullah Tuncay
Publication year - 2021
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.3323
Subject(s) - sepsis , genotype , enos , allele , biology , tumor necrosis factor alpha , medicine , polymorphism (computer science) , haplotype , immunology , nitric oxide synthase , endocrinology , gene , genetics , gastroenterology , nitric oxide
Background Sepsis is a life‐threatening condition caused by a dysregulated host response to infections and is a leading cause of death in hospitalized patients. The present study aimed to elucidate the possible association between sepsis and the tumor necrosis factor ( TNF ) gene –308G/A (rs1800629) polymorphism, as well as endothelial nitric oxide synthase ( eNOS, NOS3 ) gene –786T/C (rs2070744), 4a/4b (27 bp‐VNTR in intron 4, rs61722009) and 894G/T (Glu298Asp, rs1799983) polymorphisms. Methods In total, 188 septic adult cases and 188 healthy controls were enrolled. Genomic DNAs from the controls and patients were analyzed by polymerase chain reaction and restriction fragment length polymorphism methods. Results There were significant associations between the G/G genotype and G allele of the TNF –308G/A (rs1800629) polymorphism in the sepsis group ( p < 0.001). The presence of the T/C genotype ( p = 0.002) and C allele ( p = 0.001) of the –786T/C (rs2070744) was markedly associated with an increased risk of sepsis. However, no significant associations were found with 4a/4b (27 bp‐VNTR in intron 4, rs61722009) and 894G/T (Glu298Asp, rs1799983) polymorphisms. Higher 4bGC and lower 4bTT haplotype frequencies were associated with sepsis. Conclusions Our results strongly suggest that TNF gene (−308G/A, rs1800629) and NOS3 gene –786T/C (rs2070744) polymorphisms may modify individual susceptibility to sepsis in the Turkish population.