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lncRNA MCM3AP‐AS1 inhibits the progression of colorectal cancer via the miR‐19a‐3p/FOXF2 axis
Author(s) -
Dai Wenxin,
Zeng Wangyuan,
Lee Dongwon
Publication year - 2021
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.3306
Subject(s) - chemistry , cancer research , gene knockdown , reporter gene , colorectal cancer , gene expression , cell growth , cell , microbiology and biotechnology , cancer , gene , biology , biochemistry , genetics
Background Long non‐coding RNA MCM3AP antisense RNA 1 (lncRNA MCM3AP‐AS1) has a regulatory role in the development of diverse malignancies, whereas its role and mechanism in colorectal cancer (CRC) is not yet clear. Methods The relative expression of MCM3AP‐AS1, miR‐19a‐3p and forkhead box F2 (FOXF2) mRNA in 53 cases of CRC and its adjacent normal tissues, human normal colonic mucosal cells (FHC cells) and CRC cell lines was examined by a quantitative real‐time polymerase chain reaction, and the changes of cell multiplication and migration were examined by the cell counting kit‐8 method, EdU test, and scratch‐healing test, respectively. Bioinformatics, dual‐luciferase reporter gene assay and a RNA immunoprecipitation experiment were adopted to predict and verify the relationship between MCM3AP‐AS1 and miR‐19a‐3p; bioinformatics and dual‐luciferase reporter gene assay were adopted to predict and verify the relationship between miR‐19a‐3p and FOXF2. Western blotting was executed to examine the effects of MCM3AP‐AS1 overexpression or knockdown on FOXF2 protein expression. Results MCM3AP‐AS1 expression was down‐modulated in CRC, and its dysregulation was linked to unfavorable pathological characteristics. MCM3AP‐AS1 significantly impeded the multiplication and migration of CRC cells. MCM3AP‐AS1 was recognized as a molecular sponge to suppress miR‐19a‐3p expression, and FOXF2 was a target gene of miR‐19a‐3p. MCM3AP‐AS1 positively modulated FOXF2 expression, and its biological effect was dependent the on miR‐19a‐3p/FOXF2 axis. Conclusions MCM3AP‐AS1 can inhibit CRC promoting by modulating the miR‐19a‐3p/FOXF2 axis.