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miR‐211‐5p is down‐regulated and a prognostic marker in bladder cancer
Author(s) -
Wang Weisheng,
Liu Zhiming,
Zhang Xuegang,
Liu Junning,
Gui Junqing,
Cui Maorong,
Li Yong
Publication year - 2020
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.3270
Subject(s) - gene knockdown , cell growth , microrna , cancer research , microbiology and biotechnology , apoptosis , terminal deoxynucleotidyl transferase , biology , tunel assay , chemistry , genetics , biochemistry , gene
Background The micro RNA (miRNA)/histone deacetylase 9 (HDAC9) signaling axis has been reported to be involved in initiating and developing multiple malignant tumors. In the present study, we aimed to determine whether miR‐211‐5p serves as a post‐transcriptional regulator in bladder cancer (BCa) cell proliferation and apoptosis by targeting HDAC9. Methods miRNA expression profiling of BCa tissues and para‐carcinoma tissues was screened by miRNA microarray. After transfection with miR‐211‐5p mimics or short hairpin RNA of HDAC9 (sh‐HDAC9), mRNA and protein expression was evaluated using a quantitative reverse transcription‐polymerase chain reaction and western blotting, respectively. A bioinformatics algorithm was used, and a dual‐luciferase reporter assay was performed to validate HDAC9 as a direct target of miR‐211‐5p. Cell proliferation was analyzed by the 3‐(4, 5‐dimethylthiazl2‐yl)‐2,5‐diphenyltetazolium bromide (MTT) assay. Terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling (TUNEL) detection was used to evaluate apoptosis in 5637 and T24 cells. A transwell assay was used to assess migration and invasion. Results miR‐211‐5p is down‐regulated in BCa tumor tissues and cell lines. miR‐211‐5p is identified as an independent biomarker for predicting overall survival. HDAC9 is a direct target of miR‐211‐5p, and overexpression of miR‐211‐5p represses HDAC9 protein expression in vitro . Overexpression of miR‐211‐5p or HDAC9 knockdown significantly inhibits proliferation, migration and invasion of 5637 and T24 cells, and also induces cell apoptosis. Conclusions miR‐211‐5p may play a role as a tumor suppressor and as a favourable prognostic marker in BCa.