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METTL3 polymorphisms and Wilms tumor susceptibility in Chinese children: A five‐center case–control study
Author(s) -
Lin Ao,
Zhou Mingming,
Hua RuiXi,
Zhang Jiao,
Zhou Haixia,
Li Suhong,
Cheng Jiwen,
Xia Huimin,
Fu Wen,
He Jing
Publication year - 2020
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.3255
Subject(s) - wilms' tumor , odds ratio , genotype , genotyping , taqman , medicine , confidence interval , oncology , polymerase chain reaction , gene , biology , genetics
Background Wilms tumor is a common pediatric tumor worldwide. Methyltransferase like 3 ( METTL3 ) is a core gene of the N 6 ‐methyladenosine (m 6 A) modification that widely affects the transcription of tumor‐related genes in eukaryotes. METTL3 has been extensively investigated in various tumors but not Wilms tumor. Methods We describe a five‐center case–control study with 414 patients and 1199 controls aiming to explore the associations between METTL3 polymorphisms (rs1061026 T>G, rs1061027 C>A, rs1139130 A>G and rs1263801 G>C) and Wilms tumor susceptibility. A TaqMan real‐time polymerase chain reaction was performed for genotyping. Odds ratios (ORs) and 95% confidence intervals (CIs) were reported as evaluation indicators to determine any associations. Results Referring to the preliminary analysis results, protective genotypes were identified as rs1061026 TG/GG, rs1061027 CA/AA, rs1139130 GG and rs1263801 GC/CC. The children with three protective genotypes were less likely to develop Wilms tumor than children without protective genotypes (adjusted OR = 0.68, 95% CI = 0.46–0.999, p = 0.0496). Similarly, stratified analysis of the subgroup aged > 18 months, carrying 3 or 4 protective genotypes, was a protective factor for Wilms tumor compared to carrying 0–2 protective genotypes (adjusted OR = 0.59 95% CI = 0.39–0.91, p = 0.016). However, we did not observe any other significant results. Conclusions The combined effect of METTL3 polymorphisms reduce Wilms tumor susceptibility in Chinese children. This conclusion requires further verification.