Premium
MicroRNA‐1249 targets four‐jointed box kinase 1 and reduces cell proliferation, migration and invasion of colon adenocarcinoma
Author(s) -
Dang Wen,
Zhu Zhen
Publication year - 2020
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.3183
Subject(s) - biology , microrna , cancer research , proportional hazards model , adenocarcinoma , cancer , gene , medicine , genetics
Background MiR‐1249 was demonstrated to be dysregulated and related to prognosis in cancers. It has been reported to be significantly down‐regulated in colon adenocarcinoma (COAD). The present study aimed to explore the clinical value and biological roles of miR‐1249 in the progression of COAD. Methods miRWalk was applied to predict potential targets of miR‐1249. We investigated the expression patterns of miR‐1249 and its potential target Four‐Jointed Box Kinase 1 (FJX1) in COAD samples from The Cancer Genome Atlas (TCGA) or ONCOMINE database. Kaplan–Meier with a log‐rank test was used to reveal the relationship between overall survival (OS) and miR‐1249/FJX1. The predictive ability of miR‐1249/FJX1 was investigated using univariate and multivariate Cox regression models. CCK‐8 and Transwell assays were performed to determine whether miR‐1249 was connected with cell viability, migration and invasion. A luciferase reporter assay was applied to verify the association of miR‐1249 and FJX1 as its predicted target gene. Results We predicted and confirmed FJX1 to be a target gene of miR‐1249. MiR‐1249 was down‐regulated in COAD samples and cell lines. Univariate and multivariate analysis showed that the expression of FJX1 could be regarded as independent predictor for COAD. Moreover, miR‐1249 and FJX1 were respectively the indicators of favorable and poor OS. MiR‐1249 over‐expression repressed cell growth, migration and invasion. Overexpression of FJX1 in cells treated with miR‐1249 mimic abolished the inhibitory effect of miR‐1249 on cell growth, migration and invasion. Conclusions miR‐1249 exerts a suppressive effect on cell proliferation, migration and invasion in COAD, which is possibly achieved via modulating FJX1.