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Diagnostic and prognostic significance of serum miRNA‐146‐a expression in Egyptian children with sepsis in a pediatric intensive care unit
Author(s) -
Karam Rehab A.,
Zidan Haidy E.,
Karam Nehad A.,
Abdel Rahman Doaa M.,
ElSeifi Omnia S.
Publication year - 2019
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.3128
Subject(s) - sepsis , procalcitonin , medicine , pediatric intensive care unit , tumor necrosis factor alpha , septic shock , gastroenterology , microrna , intensive care unit , pathogenesis , c reactive protein , real time polymerase chain reaction , immunology , inflammation , intensive care medicine , biology , gene , biochemistry
Background Previous studies have suggested a strong genetic effect on sepsis pathogenesis. The present study aimed to investigate the role of miRNA‐146‐a expression in pediatric sepsis. Methods The study included 55 pediatric sepsis patients and 60 control children of the same age and sex. Serum miRNA‐146‐a expression was measured using a quantitative real‐time polymerase chain reaction. C‐reactive protein, interleukin‐6, tumor necrosis factor‐α and procalcitonin levels were measured by an enzyme‐linked immunosorbent assay. The outcome of the pediatric sepsis group was determined at 28 days of follow up. Results The results obtained revealed that serum miRNA‐146‐a levels were significantly decreased in sepsis group compared to the control group. Serum level of miRNA‐146a correlated with sepsis severity, with the pediatric septic shock group having the lowest level, followed by the severe sepsis and sepsis groups. The miRNA‐146‐a level could indicate sepsis (area under curve = 0.803). Serum miRNA‐146‐a expression was negatively associated with C‐reactive protein, pro‐calcitonin, interleukin‐6 and tumor necrosis factor‐α. Patients with miRNA‐146‐a at a level lower than 0.4 had an increased mortality rate. Conclusions miRNA‐146‐a is of significant diagnostic and prognostic value in pediatric sepsis and could be used for planning therapeutic strategies.