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Targeted elimination of activated hepatic stellate cells by an anti‐epidermal growth factor‐receptor single chain fragment variable antibody‐tumor necrosis factor‐related apoptosis‐inducing ligand (scFv425‐sTRAIL)
Author(s) -
Arabpour Mohammad,
Poelstra Klaas,
Helfrich Wijnand,
Bremer Edwin,
Haisma Hidde J.
Publication year - 2014
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.2776
Subject(s) - hepatic stellate cell , tumor necrosis factor alpha , cancer research , epidermal growth factor receptor , epidermal growth factor , hepatic fibrosis , biology , receptor , chemistry , microbiology and biotechnology , fibrosis , immunology , medicine , endocrinology , biochemistry
Background Progressive liver fibrosis is the result of chronic liver injury and is characterized by the excessive accumulation of extracellular matrix that may result in liver failure. Activated hepatic stellate cells are known to play a central role in this process and their elimination is a crucial step towards the resolution and reversion of liver fibrosis. In the present study, we investigated the potential application of an anti‐epidermal growth factor receptor single chain fragment variable antibody‐tumor necrosis factor‐related apoptosis‐inducing ligand (scFv425‐sTRAIL) fusion protein in the targeted elimination of activated hepatic stellate cells. Methods Activated hepatic stellate cells (LX2 cells) were treated by adenovirus‐derived scFv425‐sTRAIL to evaluate its effect on the viability and extracellular matrix production of this type of cells. Results In vitro treatment of activated hepatic stellate cells with scFv425‐sTRAIL induced a significant reduction in viability (up to 100% reduction) and extracellular matrix production (60% reduction), yet no significant effect was observed on hepatic parenchymal cells. Blockage of the epidermal growth factor receptor (EGFR) by a monoclonal antibody significantly reduced the effectiveness of scFv425‐sTRAIL in activated hepatic stellate cells, whereas a reduced effectivity was also observed after inhibition of the caspase pathway. Conclusions Evidence is presented for the successful application of the scFv425‐sTRAIL fusion protein in the targeted elimination of activated hepatic stellate cells via EGFR and simultaneous activation of the caspase pathway. scFv425‐sTRAIL may thus represent a new therapeutic compound against liver fibrosis. Copyright © 2014 John Wiley & Sons, Ltd.