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Targeted transgene insertion into the AAVS1 locus driven by baculoviral vector‐mediated zinc finger nuclease expression in human‐induced pluripotent stem cells
Author(s) -
Tay Felix Chang,
Tan Wee Kiat,
Goh SalLee,
Ramachandra Chrishan J. A.,
Lau CiaHin,
Zhu Haibao,
Chen Can,
Du Shouhui,
Phang Rui Zhe,
Shahbazi Mohammad,
Fan Weimin,
Wang Shu
Publication year - 2013
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.2745
Subject(s) - transgene , biology , zinc finger nuclease , induced pluripotent stem cell , expression cassette , embryonic stem cell , microbiology and biotechnology , genetics , stem cell , transcription activator like effector nuclease , gene targeting , zinc finger , genome editing , gene , genome , transcription factor , recombinant dna , vector (molecular biology)
Background The AAVS1 locus is viewed as a ‘safe harbor’ for transgene insertion into human genome. In the present study, we report a new method for AAVS1 targeting in human‐induced pluripotent stem cells (hiPSCs). Methods We have developed two baculoviral transduction systems: one to deliver zinc finger nuclease (ZFN) and a DNA donor template for site‐specific gene insertion and another to mediate Cre recombinase‐mediated cassette exchange system to replace the inserted transgene with a new transgene. Results Our ZFN system provided the targeted integration efficiency of a Neo‐EGFP cassette of 93.8% in G418‐selected, stable hiPSC colonies. Southern blotting analysis of 20 AASV1 targeted colonies revealed no random integration events. Among 24 colonies examined for mono‐ or biallelic AASV1 targeting, 25% of them were biallelically modified. The selected hiPSCs displayed persistent enhanced green fluorescent protein expression and continued the expression of stem cell pluripotency markers. The hiPSCs maintained the ability to differentiate into three germ lineages in derived embryoid bodies and transgene expression was retained in the differentiated cells. After pre‐including the loxP ‐docking sites into the Neo‐EGFP cassette, we demonstrated that a baculovirus‐Cre/loxP system could be used to facilitate the replacement of the Neo‐EGFP cassette with another transgene cassette at the AAVS1 locus. Conclusions Given high targeting efficiency, stability in expression of inserted transgene and flexibility in transgene exchange, the approach reported in the present study holds potential for generating genetically‐modified human pluripotent stem cells suitable for developmental biology research, drug development, regenerative medicine and gene therapy. Copyright © 2013 John Wiley & Sons, Ltd.