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Glutathione‐sensitive RGD‐poly(ethylene glycol)‐SS‐polyethylenimine for intracranial glioblastoma targeted gene delivery
Author(s) -
Lei Yang,
Wang Jing,
Xie Cao,
Wagner Ernst,
Lu Weiyue,
Li Ying,
Wei Xiaoli,
Dong Jibin,
Liu Min
Publication year - 2013
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.2726
Subject(s) - polyethylenimine , gene delivery , transfection , chemistry , microbiology and biotechnology , peg ratio , ethylene glycol , pegylation , viability assay , cytotoxicity , reporter gene , in vivo , in vitro , glutathione , biophysics , biochemistry , polyethylene glycol , gene expression , biology , gene , organic chemistry , enzyme , finance , economics
Background Reductively reversible and hydrolytically degradable cationic polymers have been used as gene delivery systems. The present study aimed to enhance the low transfection efficiency caused by PEGylation by taking advantage of a nonviral vector containing a disulfide linkage. Methods The novel reducible targeted gene vector c(RGDyK)‐poly(ethylene glycol)‐SS‐polyethylenimine (RGD‐PEG‐SS‐PEI), representing a combination of RGD‐PEG with PEI through a disulfide linkage, was synthesized and its reduction‐sensitivity was tested in the presence of glutathione. The RGD‐PEG‐SS‐PEI/pDNA complexes were formed and their stability was evaluated by agarose gel electrophoresis in both phosphate‐buffered saline and Dulbecco's modified Eagle's medium with 10% serum. In vitro transfection efficiency and cell viability assay of the different polymers was performed for U87 cells using pEGFP‐N2 and pGL 4.2 reporter gene systems. RGD‐PEG‐SS‐PEI/pDsRED‐N1 and RGD‐PEG‐PEI/pDsRED‐N1 complexes were injected intravenously into the U87 cell‐bearing nude mice via their tail vein to investigate in vivo gene expression. Results RGD‐PEG‐SS‐PEI has been synthesized successfully and its reduction‐sensitivity was confirmed in the presence of glutathione. The RGD‐PEG‐SS‐PEI/pDNA complexes demonstrated good stability in both conditions. In comparison with mPEG‐PEI/pDNA for gene delivery, the RGD‐PEG‐SS‐PEI/pDNA complex provided improved levels of transfection efficiency and reduced cytotoxicity when tested in U87 cells in vitro , and also enhanced levels of gene expression in the brains of intracranial U87 glioblastoma‐bearing mice as demonstrated using dsRed gene transfer and bioimaging in vivo . Conclusions The results of the present study suggest that RGD‐PEG‐SS‐PEI represents a promising candidate for further study in glioblastoma and combined gene therapies. Copyright © 2013 John Wiley & Sons, Ltd.