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Erratum: Lentiviral vector‐mediated knockdown of the neuroglycan 2 proteoglycan or expression of neurotrophin‐3 promotes neurite outgrowth in a cell culture model of the glial scar
Author(s) -
Eleanor M Donnelly,
Padraig M Strappe,
Lisa M McGinley,
Nicolas N Madigan,
Elizabeth Gurtz,
Gemma E Rooney,
Anthony J Windebank,
John Fraher,
Peter,
Dockery,
Timothy O’Brien,
Siobhan S McMahon
Publication year - 2012
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.2633
Subject(s) - gene knockdown , neurite , viral vector , proteoglycan , neurotrophin , neurotrophin 3 , cell culture , microbiology and biotechnology , neuroscience , biology , neurotrophic factors , gene , genetics , in vitro , extracellular matrix , brain derived neurotrophic factor , recombinant dna , receptor
Background; Following spinal cord injury, a highly inhibitory environment for axonal regeneration develops. One of the main sources of this inhibition is the glial scar which is formed after injury by reactive astrocytes. The inhibitory environment is mainly due to chondroitin sulphate proteoglycans (CSPGs). Neuroglycan 2 (NG2), one of the main inhibitory CSPGs, is upregulated following spinal cord injury. Methods; siRNA was designed to target NG2 and this shRNA was cloned into a lentiviral vector. The neurotrophic factor Neurotrophin-3 (NT-3) promotes the growth and survival of developing neurites and has also been shown to aid regeneration. NT-3 was also cloned into a lentiviral vector. In vitro assessment of these vectors using a co-culture system of dorsal root ganglia (DRG) neurons and Neu7 astrocytes was carried out. The Neu7 cell line is a rat astrocyte cell line which over expresses NG2, thereby mimicking the inhibitory environment following spinal cord injury. Results/Discussion; These experiments showed that both the knockdown of NG2 via shRNA and over expression of NT-3 can significantly increase neurite growth, but that a combination of both vectors did not confer any additional benefit over the vectors used individually. These lentiviral vectors show promising potential for growth and survival of neurites in injured CNS tissue