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Mathematical modelling of the impact of haematopoietic stem cell‐delivered gene therapy for HIV
Author(s) -
Murray John M.,
Fanning Greg C.,
Macpherson Janet L.,
Evans Louise A.,
Pond Susan M.,
Symonds Geoff P.
Publication year - 2009
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.1401
Subject(s) - genetic enhancement , haematopoiesis , stem cell , immunology , viral load , antiretroviral therapy , medicine , hematopoietic stem cell , human immunodeficiency virus (hiv) , lymphocyte , t cell , virology , biology , gene , immune system , genetics
Background Gene therapy represents a new treatment paradigm for HIV that is potentially delivered by a safe, once‐only therapeutic intervention. Methods Using mathematical modelling, we assessed the possible impact of autologous haematopoietic stem cell (HSC) delivered, anti‐HIV gene therapy. The therapy comprises a ribozyme construct (OZ1) directed to a conserved region of HIV‐1 delivered by transduced HSC (OZ1+HSC). OZ1+HSC contributes to the CD4+ T lymphocyte and monocyte/macrophage cell pools that preferentially expand under the selective pressure of HIV infection. The model was used to predict the efficacy of OZ1 in a highly active antiretroviral therapy (HAART) naïve individual and a HAART‐experienced individual undergoing two structured treatment operations. In the standard scenario, OZ1+HSC was taken as 20% of total body HSC. Results For a HAART‐naïve individual, modelling predicts a reduction of HIV RNA at 1 and 2 years post‐OZ1 therapy of 0.5 log 10 and 1 log 10 , respectively. Eight years after OZ1 therapy, the CD4+ T‐lymphocyte count was 271 cells/mm 3 compared to 96 cells/mm 3 for an untreated individual. In a HAART‐experienced individual HIV RNA was reduced by 0.34 log 10 and 0.86 log 10 at 1 and 2 years. The OZ1 effect was maximal when both CD4+ T lymphocytes and monocytes/macrophages were protected from successful, productive infection by OZ1. Conclusions The modelling indicates a single infusion of HSC cell‐delivered gene therapy can impact on HIV viral load and CD4 T‐lymphocyte count. Given that gene therapy avoids the complications associated with HAART, there is significant potential for this approach in the treatment of HIV. Copyright © 2009 John Wiley & Sons, Ltd.