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Alkylcarboxylate grafting to polyethylenimine: a simple approach to producing a DNA nanocarrier with low toxicity
Author(s) -
Oskuee Reza K.,
Dehshahri Ali,
Shier Wayne T.,
Ramezani Mohammad
Publication year - 2009
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.1374
Subject(s) - polyethylenimine , zeta potential , cytotoxicity , chemistry , nanocarriers , transfection , biophysics , cationic polymerization , polymer , gene delivery , vesicle , drug carrier , combinatorial chemistry , organic chemistry , nanoparticle , in vitro , biochemistry , materials science , membrane , drug delivery , nanotechnology , biology , gene
Background Various strategies have been examined to improve both transfection efficiency and cytotoxicity of polyethylenimine (PEI), a widely used polycationic nonviral gene vector. In the present study, we sought to improve PEI transfection efficiency by combining the osmotic burst mechanism for lysing endocytotic vesicles with the lipid depletion mechanism, which was accomplished by maintaining buffering capacity at the same time as adding a lipid‐absorbing hydrophobic shell. Methods PEI was altered via the substitution of various percentages of its primary amines with carboxylate‐terminated short, moderate and long alkyl chains, by reaction with bromoacetic, 6‐bromohexanoic, 10‐bromodecanoic and 16‐bromohexadecanoic acids. Modified polymers were complexed with plasmid and the particle size and zeta potential of the polyplexes were determined. Ethidium bromide dye exclusion was used to show the DNA‐binding ability of the polymers and their transfection activity and cytotoxicity was evaluated in Neuro2A mammalian cells. Results Decreased DNA‐binding ability resulted from increases in either the degree of substitution or hydrocarbon chain length. Particle size and zeta potential measurements demonstrated that modified PEI polymers were able to form nanoparticles in the size range 60–195 nm, and surface charge decreased with an increasing degree of substitution. Higher degrees of substitution resulted in decreased cytotoxicity of polymers. Alkylcarboxylate substitution of primary amines on PEI enhanced transfection efficiencies by up to approximately five‐fold relative to underivatized PEI, with the greatest increases occurring with 6‐bromohexanoic acid derivatives at degrees of substitution below 10%. Conclusions The results obtained suggest that an appropriate balance between cationic and hydrophobic regions of alkylated PEI yields the optimal nonviral vector with high transfection efficiency and low toxicity. Copyright © 2009 John Wiley & Sons, Ltd.