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Human beta‐defensin‐3 promotes wound healing in infected diabetic wounds
Author(s) -
Hirsch Tobias,
Spielmann Malte,
Zuhaili Baraa,
Fossum Magdalena,
Metzig Marie,
Koehler Till,
Steinau HansUlrich,
Yao Feng,
Onderdonk Andrew Bruce,
Steinstraesser Lars,
Eriksson Elof
Publication year - 2009
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.1287
Subject(s) - staphylococcus aureus , medicine , wound healing , beta defensin , diabetes mellitus , defensin , colony forming unit , immune system , diabetic foot , antimicrobial , immunology , microbiology and biotechnology , innate immune system , bacteria , biology , genetics , endocrinology
Background Infected wounds present a major complication in patients with diabetes. Staphylococcus aureus is the most common single isolate in diabetic wounds. Human beta‐defensin (hBD)‐3 is antimicrobial active and appears to play a key role in the immune response. The present study aimed to analyse the effect of hBD‐3 expression in a model of infected diabetic wounds. Methods Excisional wounds were created on the backs of Yorkshire pigs and Ad5‐CMV‐hBD‐3 vectors were microseeded. Wounds were inoculated with S. aureus , covered with a polyurethane chamber and analysed for transgene expression, bacterial infection, re‐epithelialization, wound contraction, wound fluid production and blood vessel formation. Results hBD‐3‐treated wounds showed a total bacterial load of 2.1 × 10 8 colony‐forming units (CFU)/g tissue, versus 1.3 × 10 9 CFU/g tissue for controls ( p < 0.001) at day 4. At day 12, no statistical difference could be detected. Re‐epithelialization showed 75 ± 15% wound closure for hBD‐3 expressing wounds and 50 ± 16% for controls ( p < 0.01). hBD‐3 expression was in the range 15–20 ng/ml of wound fluid during day 1–4. The lower dose of 2 × 10 9 Ad5‐CMV‐hBD‐3 showed no effect, suggesting a dose dependency for hBD‐3. Conclusions In the present study, we show that hBD‐3 expression significantly promotes wound closure in S. aureus infected diabetic wounds in a preclinical large‐animal model. Furthermore, a ten‐fold reduction of bacterial growth on day 4 was detected. These findings indicate that beta‐defensin‐3 may play a major role in diabetic wound healing and wound infections. Copyright © 2008 John Wiley & Sons, Ltd.