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Gene transfer into guinea pig cochlea using adeno‐associated virus vectors
Author(s) -
Konishi Masaya,
Kawamoto Kohei,
Izumikawa Masahiko,
Kuriyama Hiromichi,
Yamashita Toshio
Publication year - 2008
Publication title -
the journal of gene medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.689
H-Index - 91
eISSN - 1521-2254
pISSN - 1099-498X
DOI - 10.1002/jgm.1189
Subject(s) - inner ear , cochlea , adeno associated virus , perilymph , tropism , viral vector , biology , genetic enhancement , tissue tropism , hair cell , organ of corti , microbiology and biotechnology , vector (molecular biology) , anatomy , virus , virology , gene , recombinant dna , biochemistry
Background Several genes are candidates for treating inner ear diseases. For clinical applications, minimally invasive approaches to the inner ear are desirable along with minimal side‐effects. Methods Adeno‐associated virus (AAV) was used as a vector into the guinea pig inner ear. Six AAV‐cytomegalovirus hybrids (AAV‐2/1, ‐2/2, ‐2/5, ‐2/7, ‐2/8 and ‐2/9) were infused into perilymph of the cochlea basal turn, an approach that could be used in cochlear implant surgery. At 7 days after injection, distribution of gene expression, hearing and morphology were evaluated. Adenoviral vector was also used to compare distributions of gene expression. Moreover, distribution of cell surface receptors of AAV in the cochlea was examined using immunohistochemistry. Results Using the perilymphatic approach, adenovirus could be transferred to mesothelial cells lining the perilymph, but not sensory cells. Conversely, all AAV serotypes displayed tissue tropism to inner hair cells, with AAV‐2/2 showing particularly efficient transfer to sensory cells. This tissue tropism of AAV could not be explained by the distribution of AAV receptors. Hearing and morphology were largely unaffected. Conclusions Our results indicate that AAV vector can be safely applied to the inner ear and AAV‐2/2 offers a good tool for transferring transgenes into sensory cells of the inner ear efficiently without toxicity. Copyright © 2008 John Wiley & Sons, Ltd.