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Identification, expression pattern, and immune response of Tim‐1 and Tim‐4 in embryos and adult medaka ( Oryzias latipes )
Author(s) -
Shen Hao,
Nibona Emile,
Xu Gongyu,
Al Hafiz Md. Abdullah,
Ke Xiaomei,
Liang Xiaoting,
Yao Qiting,
Zhong Xueping,
Zhou Qingchun,
Zhao Haobin
Publication year - 2020
Publication title -
journal of experimental zoology part b: molecular and developmental evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 63
eISSN - 1552-5015
pISSN - 1552-5007
DOI - 10.1002/jez.b.22939
Subject(s) - oryzias , biology , immune system , microbiology and biotechnology , mucin , embryo , antibody , transmembrane domain , spleen , gene , immunology , genetics , biochemistry
T‐cell immunoglobulin (Ig) and mucin domain‐containing 1 (Tim‐1) and Tim‐4 are two members of the Tim family. In mammals, Tim‐1 and Tim‐4 are proteins mainly expressed in immune cells and are associated with immune response. In the present study, medaka Oryzias latipes ' Tim‐1 ( OlTim‐1 ) and OlTim‐4 were identified and characterized using bioinformatics analyses. With the use of reverse‐transcription polymerase chain reaction, the expression profiles of OlTim‐1 and OlTim‐4 were examined in embryos and adult fish and in immune tissues following the intraperitoneal injection of stimulants. The results revealed that Ol Tim‐1 possesses a cytoplasmic region, a transmembrane region, a mucin domain, and an Ig‐like domain, while Ol Tim‐4 is composed of two Ig‐like domains and a mucin domain, but without the transmembrane region and cytoplasmic region. OlTim‐1 and OlTim‐4 expressions are detectable from the gastrula stage on, indicating that they are zygotic genes. Furthermore, OlTim‐1 and OlTim‐4 are expressed ubiquitously in the adult. Administration of immune stimulants, namely lipopolysaccharides and polyinosinic:polycytidylic acid, significantly increased the expression levels of OlTim‐1 and OlTim‐4 in the liver and intestine within 1 day and in the head, kidney, and spleen within 3 to 4 days postinjection. These results suggest that Ol Tim‐1 and Ol Tim‐4 are possibly involved in both innate and adaptive immunities.