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Aurelia aurita –Cnidarian with a prominent medusiod stage
Author(s) -
Matveev Ivan V.,
Adonin Leonid S.,
Shaposhnikova Tatiana G.,
Podgornaya Olga I.
Publication year - 2011
Publication title -
journal of experimental zoology part b: molecular and developmental evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 63
eISSN - 1552-5015
pISSN - 1552-5007
DOI - 10.1002/jez.b.21440
Subject(s) - aurelia aurita , ectoderm , biology , endoderm , mesoderm , jellyfish , microbiology and biotechnology , anatomy , embryo , embryogenesis , cellular differentiation , genetics , embryonic stem cell , gene , ecology
Abstract Aurelia aurita has a complex life cycle that consists of several stages including alternating generations of medusa and polyps, huge sexual, and tiny asexual stages. Cnidarian is thought to possess two tissue layers: endoderm (gastroderm) and ectoderm, which are separated by mesoglea in medusa. The determination of the composition of the A. aurita jellyfish mesoglea was performed. New protein “mesoglein” was determined as one of the main components of mesoglea. Mesoglein is synthesized by mesogleal cells (Mc), which are populated A. aurita mesoglea as a high molecular mass precursor. Mc are involved in the formation of noncollagenous “elastic” fibers. Deduced amino acid sequence of mesoglein contains Zona Pellucida (ZP) domain and Delta/Serrate/Lag‐2 domain. According to reverse transcription PCR, mesoglein is expressed in the mature medusa exclusively in the Mc. The sperm binding to the ZP is particularly important for successful fertilization. Antibodies against mesoglein stain the plate in the place of contact of germinal epithelium and oocyte. The structure found was named the “contact plate.” The contact plate could be the precursor of the ZP. All our data suggest that Mc and, probably, the whole mesoglea originate from the epidermis (ectoderm). Computer search for mesoglein relatives reveals Nematostella and Trichoplax proteins as predicted ORFs, indicating that ZP proteins are quite ancient purchase in the evolution. J. Exp. Zool. (Mol. Dev. Evol.) 318:1–12, 2012 . © 2011 Wiley Periodicals, Inc.