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Combined use of RNAi strategies with in vitro mouse limb bud culture
Author(s) -
Zhu Yongfei,
Zhu Jiangbo,
Wan Xuying,
Zhu Yuping,
Zhang Tianbao
Publication year - 2010
Publication title -
journal of experimental zoology part b: molecular and developmental evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 63
eISSN - 1552-5015
pISSN - 1552-5007
DOI - 10.1002/jez.b.21345
Subject(s) - limb bud , rna interference , microinjection , biology , limb development , gene , embryonic stem cell , microbiology and biotechnology , in vitro , transgene , function (biology) , rna , embryo , genetics
Methods for knocking out genes and generating transgenic animals are available to study gene functions during embryogenesis. Recently, RNA interference (RNAi), a conserved eukaryotic mechanism in which double‐stranded RNA induces sequence‐specific degradation of homologous mRNAs, has become a powerful tool for investigating gene function in cells, whole animals, and embryos. Additionally, in vitro mouse limb bud culture is available to study limb development. By microinjection, we established a new method combining the use of RNAi strategies with in vitro mouse limb bud culture to investigate the functions of various genes during embryonic limb development. Using this new method, we found that HspB10 may play a role during skeletal development of limbs. It shows that the combined use of RNAi strategies (via microinjection) with in vitro mouse limb bud culture system may become a technique for investigating gene function in limb development. J. Exp. Zool. (Mol. Dev. Evol.) 314B:412–416, 2010 . © 2010 Wiley‐Liss, Inc.