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Nodal expression and heterochrony in the evolution of dorsal–ventral and left–right axes formation in the direct‐developing sea urchin Heliocidaris erythrogramma
Author(s) -
Smith Margaret S.,
Turner F. Rudolf,
Raff Rudolf A.
Publication year - 2008
Publication title -
journal of experimental zoology part b: molecular and developmental evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 63
eISSN - 1552-5015
pISSN - 1552-5007
DOI - 10.1002/jez.b.21233
Subject(s) - sea urchin , heterochrony , paracentrotus lividus , biology , nodal , larva , echinoderm , anatomy , nodal signaling , microbiology and biotechnology , strongylocentrotus purpuratus , dorsum , morphology (biology) , evolutionary biology , zoology , embryo , embryogenesis , genetics , ecology , gastrulation , ontogeny
To understand the role of body axes in the evolution of larval form, we use the two sea urchins in the genus Heliocidaris, which have distinctly different larval morphologies. Heliocidaris tuberculata is an indirect‐developing sea urchin, which forms a pluteus larva, whereas its sister species, Heliocidaris erythrogramma, exhibits direct development and forms a nonfeeding, ovoid larva. Changes along all three larval axes underlie the differences in larval form associated with each developmental mode. Nodal signaling has recently been implicated as important in establishing the dorsal–ventral (D–V) and left–right (L–R) axes in the indirect‐developing sea urchin Paracentrotus lividus . However, because of changes in morphology and timing of morphogenetic events associated with the D–V and L–R axes, respectively, in H. erythrogramma , it was unclear whether nodal played the same roles during direct development. We show that the expression patterns and functions of nodal during H. erythrogramma development are similar to its roles in indirect‐developing sea urchins in both D–V and L–R axes formation. However, there are profound changes in gene expression downstream of nodal signaling along the D–V axis and major heterochronies in the execution of the function of nodal along the L–R axis. These highly modified events are linked to the dramatic modifications of larval morphology that have occurred during the evolution of direct development in H. erythrogramma . J. Exp. Zool. (Mol. Dev. Evol.) 310B:609–622, 2008 . © 2008 Wiley‐Liss, Inc.