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Molecular cloning, expression of Sox5 and its down‐regulation of Dmrt1 transcription in Zebrafish
Author(s) -
Gao Shang,
Zhang Tao,
Zhou Xiang,
Zhao Yang,
Li Qin,
Guo Yiqing,
Cheng Hanhua,
Zhou Rongjia
Publication year - 2005
Publication title -
journal of experimental zoology part b: molecular and developmental evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 63
eISSN - 1552-5015
pISSN - 1552-5007
DOI - 10.1002/jez.b.21053
Subject(s) - zebrafish , doublesex , transcription factor , biology , promoter , cloning (programming) , genetics , gene , microbiology and biotechnology , gene expression , rna , rna splicing , computer science , programming language
The doublesex and mab‐3 ‐related transcription factor 1 ( Dmrt1 ) is the founding member of a family of DM domain genes, involved in gonadogenesis. Here we report the cloning of zebrafish Sox5 and use real time PCR to show that its expression increases while expression of Dmrt1 decreases during embryogenesis. Characterization of the proximal promoter of zebrafish Dmrt1 revealed two positive and two negative regulatory regions and a Sox5 ‐binding site. Co‐transfection of dmrt1 (with or without the Sox ‐binding site), driving an EGFP reporter and Sox5 showed further that Sox5 bound the Dmrt1 promoter and inhibited Dmrt1 expression. This antagonistic partnership between Dmrt1 and Sox5 suggests a potential transcriptional regulatory mechanism for Dmrt1 in early embryogenesis. J. Exp. Zool. (Mol. Dev. Evol.) 304B, 2005. © 2005 Wiley‐Liss, Inc.