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Geographic variability in expression of the sex‐linked AAT–1 gene of the bell‐ring frog, Buergeria buergeri
Author(s) -
Sumida Masayuki,
Ohta Shigeru,
Atsumi Shigeru,
Fujii Tamotsu
Publication year - 2004
Publication title -
journal of experimental zoology part b: molecular and developmental evolution
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.823
H-Index - 63
eISSN - 1552-5015
pISSN - 1552-5007
DOI - 10.1002/jez.b.20011
Subject(s) - heterogametic sex , biology , w chromosome , genetics , sex linkage , population , chromosome , y chromosome , gene , x chromosome , karyotype , demography , sociology
Abstract Cytological observation and artificial crossing experiments were used to examine the geographic differences in the sex‐determining mechanism and mode of inheritance of the sex‐linked AAT – 1 gene in the bell‐ring frog, Buergeria buergeri . The AAT–1 phenotypes were also examined by allozyme analysis using field‐caught females and males collected from 19 populations from the Honshu, Shikoku, and Kyushu islands of Japan, in order to comprehensively elucidate the geographic variability in the expression of the sex‐linked AAT – 1 gene of B. buergeri . The results showed that the Aomori population of B. buergeri from the northern end of Honshu was female heterogametic in sex determination, that chromosome No. VII was a sex chromosome of the ZZ/ZW type, and that the sex‐linked AAT – 1 gene was expressed on both the Z and W chromosomes. This mode of AAT – 1 expression in the Aomori population was different from that in the Hiroshima population from western Honshu, in which the AAT – 1 gene was expressed on the Z chromosome but not on the W chromosome. The results also showed that there was no differentiation among populations in the expression of the AAT – 1 genes on the Z chromosome, whereas two populations, the Hiroshima and Aomori frogs, exhibited distinct modes of expression of the AAT – 1 gene on the W chromosome. These two modes of expression may be widely distributed in western and eastern Japan, and coexist in the central part of Honshu. J. Exp. Zool. (Mol. Dev. Evol.) 302B: 182–194, 2004 . © 2004 Wiley‐Liss, Inc.