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Identification and expression profile of Delta‐GST in black tiger shrimp ( Penaeus monodon ) exposed to aflatoxin B1 (AFB1)
Author(s) -
Wang Yun,
Duan YaFei,
Wang Jun,
Zhou ChuanPeng,
Jiang ShiGui,
Lin HeiZhao,
Huang JianHua
Publication year - 2019
Publication title -
journal of experimental zoology part a: ecological and integrative physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.834
H-Index - 11
eISSN - 2471-5646
pISSN - 2471-5638
DOI - 10.1002/jez.2311
Subject(s) - penaeus monodon , hepatopancreas , biology , shrimp , aflatoxin , microbiology and biotechnology , gene , complementary dna , gene expression , biochemistry , fishery , food science
Delta GST is an insect‐specific class and a prominent class of the glutathione S ‐transferases family that is involved in xenobiotic detoxification and antioxidant defense. The full‐length complementary DNA of delta‐class GST from Penaeus monodon ( PmDeltaGST ; 839 bp long with a 657 bp coding region) was cloned. The encoded polypeptide of 218 amino acids had a predicted molecular mass of 24.30 kDa. Sequence homology and phylogenetic analysis showed that PmDeltaGST was significant similarity to GST genes in crustaceans and insects. Tissue expression profile analysis by quantitative real‐time reverse‐transcription polymerase chain showed that PmDeltaGST was constitutively expressed in all the examined tissues, with the highest expression in hepatopancreas and intestine and the weakest expression in ovary. PmDeltaGST messenger RNA expression and protein levels in hepatopancreas was significantly increased at 14 days postexposure of aflatoxin B1 (AFB1), keeping on the high level at 28 days, but decreased at 56 days. The results suggested that PmDeltaGST was involved in the response to AFB1 exposure.