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Evaluation of the sequence method as a tool to assess spontaneous baroreflex in reptiles
Author(s) -
Filogonio Renato,
Orsolini Karina F.,
Castro Samanta A.,
Oda Gustavo M.,
Rocha Gabriella C.,
Tavares Driele,
Abe Augusto S.,
Leite Cléo A. C.
Publication year - 2019
Publication title -
journal of experimental zoology part a: ecological and integrative physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.834
H-Index - 11
eISSN - 2471-5646
pISSN - 2471-5638
DOI - 10.1002/jez.2273
Subject(s) - baroreflex , vasoactive , blood pressure , sequence (biology) , heartbeat , heart rate , medicine , biology , computer science , genetics , computer security
The sequence method is an alternative to the traditional pharmacological approach (i.e., the Oxford technique) used to calculate baroreflex gain ( G ) in mammals. Although the sequence method assesses baroreflex by measuring spontaneous events of blood pressure regulation, the pharmacological method relies on the injection of vasoactive drugs that impact the baroreflex mechanism itself. The sequence method might be relevant for dynamic measurement of baroreflex modulation but it was never validated for vertebrates with low heart rate. Hence, we tested the sequence method in three species of reptiles and compared the results with those provided by the classic pharmacological method. G was similar between both methods and values correlated when parameters for the sequence method were set at delay 0 or 1 (i.e., the baroreflex system responds immediately to blood pressure changes or after 1 heartbeat). Calculation of the baroreflex effectiveness index was adequate at a minimum of 300 cycles and a delay of 1 for the three species. Therefore, the sequence method has been validated to investigate baroreflex regulation in reptiles, enabling studies during dynamic alterations in homeostasis.