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Structural and functional characterization of peptide YY on feeding in Schizothorax davidi
Author(s) -
Zhou Chaowei,
Lei Luo,
Yuan Dengyue,
Deng Xingxing,
Ye Hua,
Luo Hui,
Fang Jiayang,
Yang Minmin,
Li Yan,
Zhang Chi,
Zhou Jianshe,
Wang Jian,
Zeng Benhe,
Zhu Chengke,
Li Baohai,
Zheng Zonglin
Publication year - 2018
Publication title -
journal of experimental zoology part a: ecological and integrative physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.834
H-Index - 11
eISSN - 2471-5646
pISSN - 2471-5638
DOI - 10.1002/jez.2166
Subject(s) - peptide yy , endocrinology , medicine , neuropeptide y receptor , biology , hypothalamus , open reading frame , neuropeptide , peptide sequence , receptor , gene , biochemistry
Abstract Several studies have demonstrated that the neuropeptide peptide YY (PYY) plays an important role in feeding in mammals and fish. However, thus far, the feeding regulation function of PYY in Schizothorax davidi has not been well understood. Here, we identified the full‐length cDNA sequence of PYY in S. davidi for the first time. S. davidi PYY contains 803 bp nucleotides including a 328 bp 3′ untranslated region (UTR), a 181 bp 5′ UTR, and a 294 bp open reading frame encoding a peptide of 97 amino acids. S. davidi PYY expression was observed in almost all tissues, with the highest expression detected in the hypothalamus. PYY mRNA expression in the hypothalamus was significantly elevated after a meal ( P < 0.01), and significantly decreased after fasting ( P < 0.01). PYY expression levels were increased sharply following refeeding after 9 days ( P < 0.01), suggesting that it might function as a satiety factor in S. davidi .